Nutzung von 1-Naphthalinsulfonsäure als Schwefelquelle für die Grünalge Scenedesmus obliquus im Vergleich mit Sulfat

Luther, M.

Book/Report

FZJ-2018-03063

Nutzung von 1-Naphthalinsulfonsäure als Schwefelquelle für die Grünalge Scenedesmus obliquus im Vergleich mit Sulfat

Luther, M. (Corresponding author)FZJ*

1988
Kernforschungsanlage Jülich, Verlag Jülich

Jülich : Kernforschungsanlage Jülich, Verlag, Berichte der Kernforschungsanlage Jülich 2236, IX, 211 p. (1988)

Please use a persistent id in citations: http://hdl.handle.net/2128/18587

Report No.: Juel-2236

Abstract: Naphthalenesulfonic acids are mainly used for the manufacture of dyes and tensides for the texile industry. As rather persistent xenobiotics, they are not or only slowly degradated in wastewater treatment plants. Up to 10% of the total organic carbon in the river Rhine results from sulfonic acids. In batch experiments, it is shown, that the green alga Scenedesmus obliquus was able to use 1-naphthalenesulfonic acid as sulfur source, even in the presence of moderate concentrations of sulfate. At higher sulfate concentrations, the uptake and use of the naphthalenesulfonic acid was reduced largerly. In continous culture ( tubidostat ), the growth of Scenedesmus obliquus with 1-naphthalenesulfonic acid was extremely slow compaired with growth obtained with sulfate as sole sulfur source. Growth rates were higher at lower pH-values and since the naphthalenesulfonic acid uptake is coupled with the growth rate, higher naphthalene sulfonic acid removal rates were obtained. The removal efficiency of approximately 14% could be increased to 35% with feedback algal biomass in a specially constructed chemostat. With radioactive labelled naphthalenensulfonic acid, it could be shown that Scenedesmus obliquus is able to take up naphthalenesulfonic acid without any adaptation and removes the sulfonic acid group from the molecule as sulfur source, leaving the naphthalene ring undegradated. Cell-free extracts of Scenedesmus obliquus showed higher desulfonation rates compaired to the in-vitro experiments. Desulfonation was catalysed by enzymes which needed NAD as cofactor. In cell-free extract, sulfite was found as the desulfonation product, beside 1-naphthol, 1,4-tetralon, and 1,4-naphthochinone as organic metabolites.

Contributing Institute(s):